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KMID : 1134819990280010240
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Journal of the Korean Society of Food Science and Nutrition
1999 Volume.28 No. 1 p.240 ~ p.245
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Anticancer Effect of Doenjang in in vitro Sulforhodamine B(SRB) Assay
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Lim Sun-Young
Park Kun-Young
Lee Sook-Hee
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Abstract
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Growth inhibitory effect of doenjang(Korean soypaste) methanol extracts in SRB assay using AGS human gastric adenocarcinoma cell, Hep 3B human hepatocellular carcinoma cell and HT-29 human colon cancer cell was studied. The treatment of doenjang methanol extracts(2㎎/assay) to the AGS, Hep 3B and HT-29 cancer cells inhibited the growth of the cancer cells by 55%, 60%, and 71%, respectively. Doenjang methanol extracts exhibited the highest inhibitory effect among other soybean fermented foods and original materials in the SRB assay. In addition, to separate active compounds of doenjang methanol extracts, we fractionated the doenjang with hexane, methanol, dichloromethane, ethylacetate and butanol. Growth inhibitory effect on the AGS, Hep 3B, HT-29 and MG-63 cancer cells was the highest in the fractions of dichloromethane and ethylacetate among other solvent fractions of the doenjang. These results showed that some compounds contained in the fractions of dichloromethane and ethylacetate might playa role on the anticanceric effect of doenjang.
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KEYWORD
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donjang, SRB assay, human cancer cells
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